Klf4 has an unexpected protective role in perivascular cells within the microvasculature
Haskins, Ryan, Experimental Pathology - School of Medicine, University of Virginia
Owens, Gary, Department of Molecular Phys and Biological Physics, University of Virginia
Recent smooth muscle cell (SMC) lineage tracing studies have revealed that SMCs undergo remarkable changes in phenotype during the development of atherosclerosis. Of major interest, this work demonstrated that Klf4 in SMCs is detrimental for overall lesion pathogenesis in that SMC-specific conditional knockout of Klf4 resulted in smaller, more stable lesions that exhibited marked reductions in the numbers of SMC-derived macrophage-like and mesenchymal stem cell-like cells. However, since the clinical consequences of atherosclerosis typically occur well after our reproductive years, we sought to identify beneficial KLF4-dependent SMC functions that were likely to be evolutionarily conserved. Herein the hypothesis that Klf4 dependent SMC transitions play an important role in tissue repair following injury was tested.
Utilizing SMC-specific lineage tracing mice +/- simultaneous SMC-specific conditional knockout of Klf4, we demonstrate that SMCs in the remodeling heart following ischemia-reperfusion injury (IRI) express KLF4 and transition to a Klf4 dependent macrophage-like state and a Klf4 independent myofibroblast-like state. Moreover, SMC-Klf4 knockout mice had exacerbated heart failure following IRI. Surprisingly, significant cardiac dilation was observed in SMC-Klf4 knockout mice prior to IRI. This cardiac dilation was accompanied by a reduction in peripheral resistance, as evidenced by a reduction in blood pressure, an increase in blood flow, and a larger passive diameter of mesenteric resistance arteries as measured by pressure myography. KLF4 ChIP-Seq analysis on mesenteric vascular beds identified potential baseline SMC KLF4 target genes in numerous pathways previously shown to be important for perivascular cell investment including PDGF and FGF. Moreover, microvascular tissue beds in SMC-Klf4 knockout mice had gaps in lineage traced SMC coverage along the resistance arteries and exhibited increased permeability. Taken together, these results provide novel evidence that Klf4 has a critical maintenance role within microvascular SMCs, including being required for normal SMC function and coverage of resistance arteries.
PHD (Doctor of Philosophy)
smooth muscle cell, Klf4
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