Online Archive of University of Virginia Scholarship
Measurements of Dopamine in Drosophila using Fast-Scan Cyclic Voltammetry975 views
Author
Vickrey, Trisha Lynette, Department of Chemistry, University of Virginia
Advisors
Venton, Jill, Department of Chemistry, University of Virginia
Cafiso, David, Department of Chemistry, University of Virginia
Columbus, Linda, Department of Chemistry, University of Virginia
Demas, James, Department of Chemistry, University of Virginia
Abstract
Drosophila melanogaster, the fruit fly, is homologous to mammals in primitive neurobiology making it an advantageous model system in which to study the dynamics of dopamine regulation. However, there are few methods for measuring real-time release of dopamine in the intact fly nervous system because the size of the central nervous system (CNS) is so small. My dissertation research overcomes critical instrumentation barriers to develop the first real-time detection method for dopamine release in the fruit fly. Two methods using fast-scan cyclic voltammetry were developed to characterize dopamine homeostasis in an intact Drosophila CNS. In the first method, stimulated release was measured in Drosophila genetically modified to specifically express Channelrhodopsin2 (ChR2), a blue-light activated cation channel, in only dopaminergic neurons. In the second method, a micropipette back-filled with dopamine was implanted 15-20 µm away from a carbon-fiber microelectrode and clearance measured after dopamine was pressure ejected into the CNS. These novel methods open up the fly as a model system for studying basic mechanisms of neurotransmission.
Note: Abstract extracted from PDF text
Degree
PHD (Doctor of Philosophy)
Keywords
Dopamine; fruit fly
Language
English
Rights
All rights reserved (no additional license for public reuse)
Vickrey, Trisha Lynette. Measurements of Dopamine in Drosophila using Fast-Scan Cyclic Voltammetry. University of Virginia, Department of Chemistry, PHD (Doctor of Philosophy), 2012-07-01, https://doi.org/10.18130/V3JG2V.
