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An Analysis of Spindle Microtubule Interactions Mediated by the Ndc80 Complex, Cep57R and Cep57421 views
Author
Tooley Jr., John Gerald, Department of Cell Biology, University of Virginia
Abstract
During mitosis, kinetochores link centromeric DNA to spindle microtubules to facilitate accurate segregation of the replicated genome. Spindle poles also must form stable microtubule attachments to generate the opposing forces required for the depolymerization-coupled movement of chromosomes during anaphase. Kinetochores contain between 80-100 proteins, but the mechanism by which this macromolecular structure couples to microtubules remains unknown. Models invoking a kinetochore sleeve, a sliding ring and a fibrillar coupler have been proposed to explain how the kinetochore harnesses the energy released by a depolymerizing microtubule to power chromosome movements. In this dissertation we test two of these models through extensive study of two proteins thought to function as key kinetochore couplers.
Note: Abstract extracted from PDF text
Degree
PHD (Doctor of Philosophy)
Keywords
Mitosis; DNA; cell; biology
Language
English
Rights
All rights reserved (no additional license for public reuse)
Tooley Jr., John Gerald. An Analysis of Spindle Microtubule Interactions Mediated by the Ndc80 Complex, Cep57R and Cep57. University of Virginia, Department of Cell Biology, PHD (Doctor of Philosophy), 2011-07-01, https://doi.org/10.18130/V3V863.