Online Archive of University of Virginia Scholarship
A structure and function analysis of the Drosophila tissue polarity gene: frizzled218 views
Author
Jones, Katherine Halbert, Department of Biology, University of Virginia
Advisors
Adler, Paul N. , Department of Biology, University of Virginia
Cronmiller, Claire R. , Department of Biology, University of Virginia
Wright, Theodore R. F. , Department of Biology, University of Virginia
Hirsh, Jay, AS-Biology, University of Virginia
Smith, M, MD-MICR Microbiology, University of Virginia
Abstract
<i>Frizzled (fz)</i> is one of several <i>Drosophila</i> genes required to establish the polarity of the bristles and hairs that decorate the cuticle of the fly. <i>fz</i> has separately mutable cell non-autonomous and cell autonomous functions that are revealed in mosaic analysis of the wing. The predicted topology of <i>fz</i> places <i>fz</i> in the seven transmembrane-spanning class of integral membrane proteins, most of which are G protein-coupled receptors. I have taken evolutionary and structure-function approaches to define the <i>fz</i> protein domains that are required for intercellular and intracellular polarity signalling functions. I report here that the <i>Drosophila virilis fz</i> homolog is 92% identical to that of <i>Drosophila melanogaster</i>, and that this homolog is functional in D. <i>melancgaster</i> transgenic flies. In addition, sequence analysis of several cell non-autonomous and cell autonomous <i>fz</i> alleles demonstrates that while the non-cell autonomous alleles map throughout the protein, the cell autonomous alleles all map to a proline residue within the first putative cytoplasmic domain.
Note: Abstract extracted from PDF file via OCR.
Degree
PHD (Doctor of Philosophy)
Language
English
Rights
All rights reserved (no additional license for public reuse)
Jones, Katherine Halbert. A structure and function analysis of the Drosophila tissue polarity gene: frizzled. University of Virginia, Department of Biology, PHD (Doctor of Philosophy), 1995-01-01, https://doi.org/10.18130/v3-d1n7-sk29.
