An Inexpensive Air Stream Temperature Controller and its use to facilitate temperature-controlled behavior in Drosophila
Sangston, Ryan, Biology - Graduate School of Arts and Sciences, University of Virginia
Hirsh, Jay, AS-Biology (BIOL), University of Virginia
Controlling the environment of an organism has many biologically relevant applications. Temperature-dependent inducible biological reagents have proven invaluable for elucidating signaling cascades and dissection of neural circuits. Here we develop a simple and affordable system for rapidly changing temperature in a chamber housing adult Drosophila melanogaster. Utilizing flies expressing the temperature-inducible channel dTrpA1 in dopaminergic neurons we show rapid and reproducible changes in locomotor behavior. This device should have wide application to temperature-modulated biological reagents.
MS (Master of Science)
Dopamine, Drosophila, Thermogenetics, Locomotor Behavior, Inducible, TrpA1