Online Archive of University of Virginia Scholarship
An Inexpensive Air Stream Temperature Controller and its use to facilitate temperature-controlled behavior in Drosophila151 views
Author
Sangston, Ryan, Biology - Graduate School of Arts and Sciences, University of Virginia0000-0001-9457-9014
Advisors
Hirsh, Jay, AS-Biology (BIOL), University of Virginia
Abstract
Controlling the environment of an organism has many biologically relevant applications. Temperature-dependent inducible biological reagents have proven invaluable for elucidating signaling cascades and dissection of neural circuits. Here we develop a simple and affordable system for rapidly changing temperature in a chamber housing adult Drosophila melanogaster. Utilizing flies expressing the temperature-inducible channel dTrpA1 in dopaminergic neurons we show rapid and reproducible changes in locomotor behavior. This device should have wide application to temperature-modulated biological reagents.
Sangston, Ryan. An Inexpensive Air Stream Temperature Controller and its use to facilitate temperature-controlled behavior in Drosophila. University of Virginia, Biology - Graduate School of Arts and Sciences, MS (Master of Science), 2022-07-29, https://doi.org/10.18130/kedv-wx12.
